IDM Events

IDM Departmental Calendar

Event
Mon 10/21/2019 1:00PM - 2:00PM
IDM Seminar Series
Presentation by: Subramanian Thothathri & Jason Yeung IDM Seminar Series
Presentation by: Subramanian Thothathri & Jason Yeung
Mon 10/21/2019 1:00PM - 2:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Tue 10/22/2019 5:00PM - 8:30PM
MidAtlantic AIDS Education and Training Center Conference
Erie HIV Update: Reducing Stigma and Improving Sexual Health MidAtlantic AIDS Education and Training Center Conference
Erie HIV Update: Reducing Stigma and Improving Sexual Health
Tue 10/22/2019 5:00PM - 8:30PM
Outside Conference and Meeting Spaces

This evening dinner update, in collaboration with the Erie Department of Health and Erie HIV Task Force, will be held at the Bayfront Convention Center.  The presenters will discuss Sexual Health History Taking and Sexual Health and Stigma, followed by a panel of local providers.


Outside Conference and Meeting Spaces
Wed 10/23/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 10/23/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health
Wed 10/23/2019 12:00PM - 1:00PM
IDM MPH Journal Club
Practicum Panel: 1st & 2nd Years IDM MPH Journal Club
Practicum Panel: 1st & 2nd Years
Wed 10/23/2019 12:00PM - 1:00PM
A216 Public Health


A216 Public Health
Mon 10/28/2019 12:00PM - 1:00PM
IDM Seminar Series
Presentations by: Byron Chuan & Zachary Koenig IDM Seminar Series
Presentations by: Byron Chuan & Zachary Koenig
Mon 10/28/2019 12:00PM - 1:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Wed 10/30/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 10/30/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health
Wed 10/30/2019 12:00PM - 1:00PM
IDM MPH Journal Club
Article - Metagenomic analysis of viruses in toilet waste from long distance flights --.... IDM MPH Journal Club
Article - Metagenomic analysis of viruses in toilet waste from long distance flights --....
Wed 10/30/2019 12:00PM - 1:00PM
A425 Public Health


A425 Public Health
Mon 11/4/2019 12:00PM - 1:00PM
IDM Seminar Series
Presentation by Mari Webel, PhD IDM Seminar Series
Presentation by Mari Webel, PhD
Mon 11/4/2019 12:00PM - 1:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Wed 11/6/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 11/6/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health
Wed 11/6/2019 12:00PM - 1:00PM
IDM MPH Journal Club
Article - Effect of Microglial Inflammation in Prion Disease IDM MPH Journal Club
Article - Effect of Microglial Inflammation in Prion Disease
Wed 11/6/2019 12:00PM - 1:00PM
A425 Public Health


A425 Public Health
Mon 11/11/2019 12:00PM - 1:00PM
IDM Seminar Series
Presentations by: Emily Olsen & Karsen Shoger IDM Seminar Series
Presentations by: Emily Olsen & Karsen Shoger
Mon 11/11/2019 12:00PM - 1:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Wed 11/13/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 11/13/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health
Mon 11/18/2019 12:00PM - 1:00PM
IDM Seminar Series
Presentations by: Victoria Gould & Katrina Stephenson IDM Seminar Series
Presentations by: Victoria Gould & Katrina Stephenson
Mon 11/18/2019 12:00PM - 1:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Wed 11/20/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 11/20/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health
Wed 11/20/2019 12:00PM - 1:00PM
IDM MPH Journal Club
Article - Pulmonary involvement during the Ebola Virus Disease IDM MPH Journal Club
Article - Pulmonary involvement during the Ebola Virus Disease
Wed 11/20/2019 12:00PM - 1:00PM
A425 Public Health


A425 Public Health
Wed 11/20/2019 12:00PM - 1:00PM
IDM MPH Journal Club
Article - TBA IDM MPH Journal Club
Article - TBA
Wed 11/20/2019 12:00PM - 1:00PM
A425 Public Health


A425 Public Health
Mon 12/2/2019 12:00PM - 1:00PM
IDM Seminar Series
Presentation by: Gwen Kettenburg IDM Seminar Series
Presentation by: Gwen Kettenburg
Mon 12/2/2019 12:00PM - 1:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Wed 12/4/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 12/4/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health

Recent Events

IDM Dissertation Defense

Ben Policicchio - PhD '17

Thursday 12/7 11:00AM - 1:00PM
Public Health Lecture Hall (A115)

USE OF INTEGRASE INHIBITOR TO ASSESS THE CYTOTOXIC T LYMPHOCYTE RESPONSE DURING SIMIAN IMMUNODEFICIENCY VIRUS INFECTION IN RHESUS MACAQUES

Abstract: The host immune response against human immunodeficiency virus (HIV) infection is multi-faceted, with the cytotoxic T lymphocyte (CTL) response playing a significant role in controlling the virus. However, it is uncertain what mechanism(s) these CTLs utilize to suppress virus replication. Previous research utilizing mathematical modeling and nucleoside reverse transcriptase inhibitors (NRTIs) showed that CTLs most likely have a non-cytolytic effect against infected cells, but these data are not universally accepted in the field. I hypothesized that CTLs exert the majority of their cytolytic function against infected cells containing virus that has yet to be integrated, due to minimal viral cytopathic effects at this stage. To test this hypothesis, I modeled the impact of CD8+ T cells through experimental CD8+ cell depletion on the pre- and post-integration stages of simian immunodeficiency virus (SIV) infection on both plasma viral load and 2-LTR circles. Model predictions were tested in the pathogenic SIVmac251-infected rhesus macaques (RMs) receiving the integrase inhibitor raltegravir (RAL) monotherapy with or without CD8+ T cells. CD8+ T cell depletion was profound and lasted throughout RAL therapy, with administration resulting in an immediate and sustained suppression of CD8+ cells. CD4+ cells recovered slightly during RAL treatment. The estimated efficacy of RAL in preventing integration was 97%. We next calculated the loss rate of infected cells prior to integration and determined that this loss rate was reduced by 82% in the CD8+ cell depletion with RAL treatment group compared to the RAL monotherapy group, but also that viral production increased 2.5 times in the absence of CD8+ cells. Further, we observed that CD8+ cell depletion by itself increases 2-LTR circles, RAL monotherapy does not affect 2-LTR circle levels, and combination treatment increases 2-LTR circle levels faster than CD8 depletion alone. Upon fitting an extended viral dynamics model to the 2-LTR data, we conclude that the data best matches the hypothesis that CD8+ cells exert a killing effect on infected cells prior to integration, supporting our hypothesis. We attempted to analyze the effects of RAL intensification on 2-LTR circle dynamics, but we were unable to due to the development of resistance mutations, reinforcing the fact that in suboptimally ART-treated RMs, mutations do, indeed, occur, resulting in viral rebound during treatment. The public health significance of this dissertation is that by showing CD8+ cells kill cells containing virus prior to integration, we are aiding in the hunt for an elusive vaccine and/or cure against HIV.

Advisor: Ivona Vasile-Pandrea, MD, PhD

Last Updated On Monday, February 26, 2018 by Abby Kincaid
Created On Tuesday, November 14, 2017

Submit events and news

Enter upcoming calendar events or share your school news and announcements at publichealth.pitt.edu/submit.