IDM Events

IDM Departmental Calendar

Event
Mon 10/21/2019 1:00PM - 2:00PM
IDM Seminar Series
Presentation by: Subramanian Thothathri & Jason Yeung IDM Seminar Series
Presentation by: Subramanian Thothathri & Jason Yeung
Mon 10/21/2019 1:00PM - 2:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Tue 10/22/2019 5:00PM - 8:30PM
MidAtlantic AIDS Education and Training Center Conference
Erie HIV Update: Reducing Stigma and Improving Sexual Health MidAtlantic AIDS Education and Training Center Conference
Erie HIV Update: Reducing Stigma and Improving Sexual Health
Tue 10/22/2019 5:00PM - 8:30PM
Outside Conference and Meeting Spaces

This evening dinner update, in collaboration with the Erie Department of Health and Erie HIV Task Force, will be held at the Bayfront Convention Center.  The presenters will discuss Sexual Health History Taking and Sexual Health and Stigma, followed by a panel of local providers.


Outside Conference and Meeting Spaces
Wed 10/23/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 10/23/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health
Wed 10/23/2019 12:00PM - 1:00PM
IDM MPH Journal Club
Practicum Panel: 1st & 2nd Years IDM MPH Journal Club
Practicum Panel: 1st & 2nd Years
Wed 10/23/2019 12:00PM - 1:00PM
A216 Public Health


A216 Public Health
Mon 10/28/2019 12:00PM - 1:00PM
IDM Seminar Series
Presentations by: Byron Chuan & Zachary Koenig IDM Seminar Series
Presentations by: Byron Chuan & Zachary Koenig
Mon 10/28/2019 12:00PM - 1:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Wed 10/30/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 10/30/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health
Wed 10/30/2019 12:00PM - 1:00PM
IDM MPH Journal Club
Article - Metagenomic analysis of viruses in toilet waste from long distance flights --.... IDM MPH Journal Club
Article - Metagenomic analysis of viruses in toilet waste from long distance flights --....
Wed 10/30/2019 12:00PM - 1:00PM
A425 Public Health


A425 Public Health
Mon 11/4/2019 12:00PM - 1:00PM
IDM Seminar Series
Presentation by Mari Webel, PhD IDM Seminar Series
Presentation by Mari Webel, PhD
Mon 11/4/2019 12:00PM - 1:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Wed 11/6/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 11/6/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health
Wed 11/6/2019 12:00PM - 1:00PM
IDM MPH Journal Club
Article - Effect of Microglial Inflammation in Prion Disease IDM MPH Journal Club
Article - Effect of Microglial Inflammation in Prion Disease
Wed 11/6/2019 12:00PM - 1:00PM
A425 Public Health


A425 Public Health
Mon 11/11/2019 12:00PM - 1:00PM
IDM Seminar Series
Presentations by: Emily Olsen & Karsen Shoger IDM Seminar Series
Presentations by: Emily Olsen & Karsen Shoger
Mon 11/11/2019 12:00PM - 1:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Wed 11/13/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 11/13/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health
Mon 11/18/2019 12:00PM - 1:00PM
IDM Seminar Series
Presentations by: Victoria Gould & Katrina Stephenson IDM Seminar Series
Presentations by: Victoria Gould & Katrina Stephenson
Mon 11/18/2019 12:00PM - 1:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Wed 11/20/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 11/20/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health
Wed 11/20/2019 12:00PM - 1:00PM
IDM MPH Journal Club
Article - Pulmonary involvement during the Ebola Virus Disease IDM MPH Journal Club
Article - Pulmonary involvement during the Ebola Virus Disease
Wed 11/20/2019 12:00PM - 1:00PM
A425 Public Health


A425 Public Health
Wed 11/20/2019 12:00PM - 1:00PM
IDM MPH Journal Club
Article - TBA IDM MPH Journal Club
Article - TBA
Wed 11/20/2019 12:00PM - 1:00PM
A425 Public Health


A425 Public Health
Mon 12/2/2019 12:00PM - 1:00PM
IDM Seminar Series
Presentation by: Gwen Kettenburg IDM Seminar Series
Presentation by: Gwen Kettenburg
Mon 12/2/2019 12:00PM - 1:00PM
Public Health Lecture Hall (A115)


Public Health Lecture Hall (A115)
Wed 12/4/2019 12:00PM - 1:00PM
IDM PhD & MS Journal Club
Article - TBA IDM PhD & MS Journal Club
Article - TBA
Wed 12/4/2019 12:00PM - 1:00PM
A719 Public Health


A719 Public Health

Recent Events

IDM Dissertation Defense

Parichat Duangkhae - PhD '18: Defining Dengue Virus Infection in Human Skin

Tuesday 4/3 2:00PM - 4:00PM
Public Health Lecture Hall (A115)

DEFINING DENGUE VIRUS INFECTION IN HUMAN SKIN

Abstract: The skin is the primary site of dengue virus (DENV) replication following the bite of an infected mosquito, but the factors that contribute to productive infection in human skin and virus spread out of skin are not understood. We defined the dynamics of DENV infection in human skin explants using quantitative in situ imaging. A transient IFN-α response occurred prior to the productive DENV infection, which initially established in cells in the epidermis. DENV infected a wide variety of cell types including Langerhans cells (LC), dermal macrophages (Mϕ), dermal dendritic cells (DC), fibroblasts, mast cells, and lymphatic endothelium, but keratinocytes were the earliest and quantitatively most important target of DENV infection, contributing to 60% of overall infected skin cell over time. DENV infection led to the recruitment and infection of LC, dermal DC, and dermal Mϕ. These immune cells emigrated out of the skin in increased number as a result of infection, presumably leading to dissemination of virus. Infection of keratinocytes led to the abundant production of inflammatory mediators, most significantly IL-1β. Blocking keratinocyte-derived IL-1β reduced the infection of LC, dermal DC, and dermal Mϕ by 75-90% and decreased the total number of infected cells in epidermis and dermis by 33% and 65%, respectively. In the first demonstration of antibody-dependent enhancement of DENV infection in human skin, we showed that the presence of heterotypic DENV-immune serum enhanced the recruitment and infection of dermal Mϕ by 50-70%, and increased emigration of myeloid cells out of skin. The Aedes aegypti mosquito salivary gland extract had no impact on dermal Mϕ during DENV infection, with or without immune serum. Blocking FcγRIa and FcγRIIa strongly diminished all the antibody-mediated infection of dermal Mϕ, and decreased the number of cell emigrants, resulting in the reduction of the overall number of infected cells in the dermis by 70%, without notable changes in the epidermis. Ethnic differences in skin immune responses to DENV were observed for the first time in our study. In comparison with Caucasian skin, skin from African Americans was able to maintain the protective level of antiviral IFN-α responses for at least 48 hours. This was observed in association with less DENV replication, a reduced production of IL-1β in the epidermis, less recruitment and infection of LC and dermal Mϕ, and less cell emigration out of the skin. These findings may provide a mechanism for the epidemiologic observation that African ancestry protects against severe dengue. Our findings highlight the importance of skin and the complex interplay between resident and immune skin cell populations in DENV infection and dengue pathogenesis.

Advisor: Simon Barratt-Boyes, PhD

Defense Announcement - Duangkhae.pdf

Last Updated On Friday, March 30, 2018 by Abby Kincaid
Created On Tuesday, March 27, 2018

Submit events and news

Enter upcoming calendar events or share your school news and announcements at publichealth.pitt.edu/submit.