IDM Events

IDM Departmental Calendar

Event Category
Fri 9/20/2019 8:30AM - 12:00PM
35th Anniversary of the Pitt Men's Study IDM Event
35th Anniversary of the Pitt Men's Study
Fri 9/20/2019 8:30AM - 12:00PM
Public Health Auditorium (G23)


Public Health Auditorium (G23)
IDM
Event
Tue 10/8/2019 9:00AM - 4:30PM
PrEP Summit: Engagement, Adherence, Retention MidAtlantic AIDS Education and Training Center Conference
PrEP Summit: Engagement, Adherence, Retention
Tue 10/8/2019 9:00AM - 4:30PM
Outside Conference and Meeting Spaces


Outside Conference and Meeting Spaces
MidAtlantic AIDS Education and Training Center
Conference
Tue 10/22/2019 5:00PM - 8:30PM
Erie Evening Update: Sexual Health and Stigma MidAtlantic AIDS Education and Training Center Conference
Erie Evening Update: Sexual Health and Stigma
Tue 10/22/2019 5:00PM - 8:30PM
Outside Conference and Meeting Spaces

This evening dinner update, in collaboration with the Erie Department of Health and Erie HIV Task Force, will be held at the Bayfront Convention Center.  The presenters will discuss Sexual Health History Taking and Sexual Health and Stigma, followed by a panel of local providers.


Outside Conference and Meeting Spaces
MidAtlantic AIDS Education and Training Center
Conference

Recent Events

IDM Dissertation Defense

Diana Campbell Delucia - PhD '18: Cellular cholesterol regulation of HIV-1 trafficking during...

Thursday 3/1 11:00AM - 1:00PM
Public Health Lecture Hall (A115)

CELLULAR CHOLESTEROL REGULATION OF HIV-1 TRAFFICKING DURING MACROPHAGE-MEDIATED TRANS INFECTION

Abstract: Professional antigen presenting cells (APC: myeloid dendritic cells (DC) and macrophages (MΦ); B lymphocytes) mediate highly efficient HIV-1 infection of CD4+ T cells, termed trans infection, that could contribute to HIV-1 pathogenesis. We have previously shown that lower cholesterol content in DC and B lymphocytes is associated with a lack of HIV-1 trans infection in HIV-1 infected nonprogressors (NP). Here we assessed whether HIV-1 trans infection mediated by another major APC, MΦ, is deficient in NP due to altered cholesterol metabolism. When comparing healthy HIV-1 seronegatives (SN), rapid progressors (PR), and NP, we found that monocyte-derived MΦ from NP did not mediate HIV-1 trans infection of autologous CD4+ T cells, in contrast to efficient trans infection mediated by SN and PR MΦ. MΦ trans infection efficiency was directly associated with the number of DC-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN)-expressing MΦ. Significantly fewer NP MΦ expressed DC-SIGN. Unesterified (free) cholesterol in MΦ cell membranes was significantly lower in NP than PR, as well as virus internalization in early endosomes. Furthermore, simvastatin (SIMV), decreased the subpopulation of DC-SIGN+ MΦ, as well as MΦ cis and trans infection. Notably, SIMV decreased cell membrane cholesterol and led to lipid raft dissociation, effectively mimicking the incompetent APC trans infection environment characteristic of NP. Our data support that DC-SIGN and membrane cholesterol are central to MΦ trans infection, and a lack of these limits HIV-1 disease progression. Lastly, we identified differentially expressed miRNA in NP and PR plasma which may contribute to the altered cholesterol phenotype we have identified in NP APC. Understanding the mechanisms of HIV-1 trans infection has great public health importance. For example, targeting the ability of MΦ to drive HIV-1 dissemination in trans could significantly enhance HIV-1 therapeutic strategies.

Advisor: Giovanna Rappocciolo

Last Updated On Friday, March 30, 2018 by Abby Kincaid
Created On Monday, February 26, 2018

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