This evening dinner update, in collaboration with the Erie Department of Health and Erie HIV Task Force, will be held at the Bayfront Convention Center. The presenters will discuss Sexual Health History Taking and Sexual Health and Stigma, followed by a panel of local providers.
Abstract: Prostratin has been applied as a promising latency reversing agent for the eradication of HIV-1 latent CD4+ T cell reservoirs, yet the molecular mechanism of this reactivation remains unclear. Here, we design a novel chemical biology approach revealing that PKC-Mek pathway is essential for prostratin-induced reactivation of latent HIV-1 provirus. First, 7 different Mek inhibitors were identified by the approach that constantly impaired prostratin-induced reactivation. Second, PKC inhibitors, Raf inhibitors, and Erk inhibitors showed different level of inhibition in the latency reversal induced my prostratin. Consistent with this observation, we found that prostratin-induced latency reversal was involved with the kinase activation cascade of PKC-Mek pathway. In addition, we identified several kinase inhibitors that activated HIV-1 latent genes in a significant level individually, including CUDC-101, XMD8-92, PD173074, Quizartinib, and CUDC-907. Therefore, this chemical biology approach provides new methods of revealing the molecular mechanism of LRAs into the HIV-1 eradication research.
Last Updated On Thursday, April 27, 2017 by Malenka, Judith Ann
Created On Tuesday, April 18, 2017