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Yue Chen, PhD

Assistant Professor, Infectious Diseases and Microbiology

Contact

2138 Parran Hall, 130 DeSoto Street, Pittsburgh, PA 15261
R-znvy: pural@cvgg.rqh
Primary Phone: 967-179-0843


Personal Statement

Exploring the mechanism of HCV spontaneous clearance in HIV-1 infected individuals. For some unclear reasons, HCV infections are spontaneously cleared in certain HIV/HCV coinfected individuals, whereas in majority of HIV/HCV coinfected   individuals, HCV infections progress to chronicity. To explore the mechanism of such difference, plasma RNA from HIV-1 infected subjects with HCV spontaneous clearance or chronic infection have being comprehensively analyzed following next generation sequencing of plasma RNA to elucidate the mechanism of HCV spontaneous clearance.

Exploring the mechanism of HIV-1 transmission through human mucosal surfaces. It is unclear how HIV-1 crosses the intact epithelial layers to infect sub-mucosal CD4 positive cells in ectocervical and rectal mucosal area. To investigate the mechanism of HIV-1 transmission, normal human colon and cervical tissue biopsies are exposed to HIV-1 or control supernatant in an organ culture model. The effect of HIV-1 exposure is evaluated by monitoring the changes of morphology, tight junction protein expression and cytokine gene expression in epithelial layers of mucosal surface following HIV-1 exposure. The study will provide important information regarding the mechanism of HIV transmission through mucosal regions. 


In cancer patients, tumor cells are rapidly dividing, constantly releasing tumor DNA into the blood. Recent advances in next-generation sequencing have made it possible to sequence circulating tumor DNA (ctDNA) in blood plasma with high sensitivity and specificity, termed “liquid biopsy”. This will have a key role in clinically assisting early diagnosis, longitudinal analyses of tumor evolution and personalized therapeutic intervention. We hypothesize that there are sufficient ctDNA in the plasma of HIV-1 infected MACS subjects at least 6 months prior to development of AIDS- associated cancers that can be measured and used as biomarkers for early diagnosis and personalized therapeutic intervention of AIDS related cancers. To detect the ctDNA in plasma samples of AIDS- associated cancer patients before and after the cancer diagnosis, longitudinal MACS plasma samples are being used to detect ctDNA via a next-generation sequencing with an Ion AmpliSeq comprehensive cancer panel, which targets 409 tumor suppressor genes and oncogenes frequently cited and frequently mutated in cancer cells. The quantity and evolution of ctDNA will be monitored in plasma DNA. Our eventual goal is to develop a noninvasive method for detecting plasma ctDNA, which assists early diagnosis and personalized therapeutic intervention for AIDS- associated cancers.?


Pennsylvania/MidAtlantic AIDS Education and Training Center (PA/MidAtlantic AETC) provides HIV/AIDS  education, consultation, technical assistance and resource materials to health care professionals throughout Pennsylvania, Ohio, Maryland, Virginia, West Virginia, Delaware and District of Columbia. Our effort to educate and train healthcare professionals will increase the providers' capacity to provide high-quality HIV/AIDS care.
   


Education

1978-1983 | Dalian Medical University, China | MD, Medicine
1986-1989 | Xian Medical University, China | MS, Pathophysiology
1992-1998 | University o Pittsburgh, PA | PhD,  Virology
1998-1999 | Department of IDM, University of Pittsburgh | Post-doc Fellow


Teaching

IDM 2023, Microbiology Laboratory
IDM 2003, Host Response to Microbial Infection
IDM 2038, Prevention, Treatment, and Control of Global Infectious Diseases
IDM 2013, Viral Pathogenesis
IDM 2034, Control and Prevention of HIV/AIDS


Selected Publications

Shen C, Gupta P, Xu XC, Sanyal A, Rinaldo C, Seaberg E, Morgolick JB, Wolinsky S, Martinez-Maza O, Chen Y.  (2014) Transmission and evolution of Hepatitis C Virus in HCV seroconverters in HIV/HCV co-infected subjects. Virology 449:339-349.

Shridhar V, Chen Y, Gupta P.  (2014) The CD8 Antiviral Factor (CAF) can suppress HIV-1 Transcription from the Long Terminal Repeat Promoter in the Absence of Elements Upstream of the CATATAA Box. Virol J.  11:130.

Zhao G, Miao Y, Guo Y, Qiu H, Sun S, Kou Z, Yu H, Li J, Chen Y, Jiang S, Du L, Zhou Y.  (2014) Development of a heat-stable and orally delivered recombinant M2e-expressing B. subtilis spore-based influenza vaccine. Hum Vaccin Immunother. 10:3649-3658.

Lu J, Zhang S, Liu Y, Du X, Ren S, Zhang H, Ma L, Chen Y, Chen X, Shen C.  (2015) Effect of Peg-interferon α-2a combined with Adefovir in HBV postpartum women with normal levels of ALT and high levels of HBV DNA. Liver Int. 35:1692-1699.

Rossi F, Li X, Jacobson L, Levine AJ, Chen Y, Palella FJ, Margolick J, Viscidi R.  (2015)  BK virus capsid antibodies are associated with protection against subsequent development of PML in HIV-infected patients. Virology. 485:467-472.

Kuniholm MH, Ong E, Hogema BM, Koppelman M, Anastos K, Peters MG, Seaberg EC, Chen Y, Nelson KE, Linnen JM.  (2016) Acute and Chronic Hepatitis E Virus Infection in Human Immunodeficiency Virus-Infected U.S. Women. Hepatology. 63:712-720.

Sankapal S, Gupta P, Ratner D, Ding M, Shen C, Sanyal A, Stolz D, Cu-Uvin S, Ramratnam B, Chen Y.  (2016)  HIV exposure to epithelial cells in ectocervical and colon tissues induces inflammatory cytokines without tight junction disruption. AIDS Res Hum Retroviruses. [Epub ahead of print].

 


Yue  Chen
© 2017 by University of Pittsburgh Graduate School of Public Health

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