Detcho A. Stoyanovksy, PhD

Research Associate Professor, Environmental and Occupational Health

Contact

130 DeSoto Street, Room 4123, Pittsburgh, PA 15261
R-znvy: qnf66@cvgg.rqh
Primary Phone: 967-838-1465
Secondary Phone: 967-838-1485
Fax: 967-179-4816

Assistant(s): 
Lynette Clark, yipynex@cvgg.rqh, 967-179-4255

Personal Statement

My research interests are focused on the synthesis of radiation mitigators that selectively compartmentalize in the mitochondria of non-malignant cells and the mechanisms of S-(de)nitrosation of proteins. 


Education

1986    Faculty of Chemistry, Sofia University, Sofia, Bulgaria, EU-   M.S. degree in organic and in analytical chemistry.

1991    Bulgarian Academy of Sciences, Sofia, Bulgaria, EU- Ph.D. in biochemistry.



 


Selected Publications

[1] D.A. Stoyanovsky, Y.Y. Tyurina, I. Shrivastava, I. Bahar, V.A. Tyurin, O. Protchenko, S. Jadhav, S.B. Bolevich, A.V. Kozlov, Y.A. Vladimirov, A.A. Shvedova, C.C. Philpott, H. Bayir, V.E. Kagan, Iron catalysis of lipid peroxidation in ferroptosis: Regulated enzymatic or random free radical reaction?, Free Radic Biol Med, 133 (2019) 153-161. PMID:30217775


[2] A.D. Stoyanovsky, D.A. Stoyanovsky, 1-Oxo-2,2,6,6-tetramethylpiperidinium bromide converts alpha-H N,N-dialkylhydroxylamines to nitrones via a two-electron oxidation mechanism, Sci Rep, 8 (2018) 15323.PMC6193029


[3] W. Lou, H.C. Ting, C.A. Reynolds, Y.Y. Tyurina, V.A. Tyurin, Y. Li, J. Ji, W. Yu, Z. Liang, D.A. Stoyanovsky, T.S. Anthonymuthu, M.A. Frasso, P. Wipf, J.S. Greenberger, H. Bayir, V.E. Kagan, M.L. Greenberg, Genetic re-engineering of polyunsaturated phospholipid profile of Saccharomyces cerevisiae identifies a novel role for Cld1 in mitigating the effects of cardiolipin peroxidation, Biochim Biophys Acta Mol Cell Biol Lipids, 1863 (2018) 1354-1368. PMID:29935382


[4] M.J. Scott, T.R. Billiar, D.A. Stoyanovsky, N-tert-butylmethanimine N-oxide is an efficient spin-trapping probe for EPR analysis of glutathione thiyl radical, Sci Rep, 6 (2016) 38773.PMC5150238


[5] N. Yanamala, A.A. Kapralov, M. Djukic, J. Peterson, G. Mao, J. Klein-Seetharaman, D.A. Stoyanovsky, J. Stursa, J. Neuzil, V.E. Kagan, Structural re-arrangement and peroxidase activation of cytochrome c by anionic analogues of vitamin E, tocopherol succinate and tocopherol phosphate, J Biol Chem, 289 (2014) 32488-32498.PMC4239604


[6] D.A. Stoyanovsky, L.J. Sparvero, A.A. Amoscato, R.R. He, S. Watkins, B.R. Pitt, H. Bayir, V.E. Kagan, Improved spatial resolution of matrix-assisted laser desorption/ionization imaging of lipids in the brain by alkylated derivatives of 2,5-dihydroxybenzoic acid, Rapid Commun Mass Spectrom, 28 (2014) 403-412.PMC3973445


[7] D.A. Stoyanovsky, J. Jiang, M.P. Murphy, M. Epperly, X. Zhang, S. Li, J. Greenberger, V. Kagan, H. Bayir, Design and Synthesis of a Mitochondria-Targeted Mimic of Glutathione Peroxidase, MitoEbselen-2, as a Radiation Mitigator, ACS Med Chem Lett, 5 (2014) 1304-1307.PMC4266336


[8] M. Fatfat, R.A. Merhi, O. Rahal, D.A. Stoyanovsky, A. Zaki, H. Haidar, V.E. Kagan, H. Gali-Muhtasib, K. Machaca, Copper chelation selectively kills colon cancer cells through redox cycling and generation of reactive oxygen species, BMC Cancer, 14 (2014) 527.PMC4223620


[9] D.A. Stoyanovsky, M.J. Scott, T.R. Billiar, Glutathione and thioredoxin type 1 cooperatively denitrosate HepG2 cells-derived cytosolic S-nitrosoproteins, Org Biomol Chem, 11 (2013) 4433-4437.PMC3824615


[10] Y.Y. Tyurina, M.A. Tungekar, M.Y. Jung, V.A. Tyurin, J.S. Greenberger, D.A. Stoyanovsky, V.E. Kagan, Mitochondria targeting of non-peroxidizable triphenylphosphonium conjugated oleic acid protects mouse embryonic cells against apoptosis: role of cardiolipin remodeling, FEBS Lett, 586 (2012) 235-241.PMC3273856


[11] K. Thambiayya, K. Wasserloos, V.E. Kagan, D. Stoyanovsky, B.R. Pitt, A critical role for increased labile zinc in reducing sensitivity of cultured sheep pulmonary artery endothelial cells to LPS-induced apoptosis, Am J Physiol Lung Cell Mol Physiol, 302 (2012) L1287-1295.PMC3379046


[12] D.A. Stoyanovsky, A. Maeda, J.L. Atkins, V.E. Kagan, Assessments of thiyl radicals in biosystems: difficulties and new applications, Anal Chem, 83 (2011) 6432-6438. PMID:21591751


[13] D.A. Stoyanovsky, Z. Huang, J. Jiang, N.A. Belikova, V. Tyurin, M.W. Epperly, J.S. Greenberger, H. Bayir, V.E. Kagan, A manganese-porphyrin complex decomposes H(2)O(2), inhibits apoptosis, and acts as a radiation mitigator in vivo, ACS Med Chem Lett, 2 (2011) 814-817.PMC3254103


[14] H.H. Li, J. Xu, K.J. Wasserloos, J. Li, Y.Y. Tyurina, V.E. Kagan, X. Wang, A.F. Chen, Z.Q. Liu, D. Stoyanovsky, B.R. Pitt, L.M. Zhang, Cytoprotective effects of albumin, nitrosated or reduced, in cultured rat pulmonary vascular cells, Am J Physiol Lung Cell Mol Physiol, 300 (2011) L526-533.PMC3075101


[15] J. Atkinson, A.A. Kapralov, N. Yanamala, Y.Y. Tyurina, A.A. Amoscato, L. Pearce, J. Peterson, Z. Huang, J. Jiang, A.K. Samhan-Arias, A. Maeda, W. Feng, K. Wasserloos, N.A. Belikova, V.A. Tyurin, H. Wang, J. Fletcher, Y. Wang, Vlasova, II, J. Klein-Seetharaman, D.A. Stoyanovsky, H. Bayir, B.R. Pitt, M.W. Epperly, J.S. Greenberger, V.E. Kagan, A mitochondria-targeted inhibitor of cytochrome c peroxidase mitigates radiation-induced death, Nat Commun, 2 (2011) 497.PMC3557495


[16] D.A. Stoyanovsky, A. Kapralov, Z. Huang, A. Maeda, A. Osipov, C.J. Hsia, L. Ma, P.M. Kochanek, H. Bayr, V.E. Kagan, Unusual peroxidase activity of polynitroxylated pegylated hemoglobin: Elimination of H(2)O(2) coupled with intramolecular oxidation of nitroxides, Biochem Biophys Res Commun, 399 (2010) 139-143. PMID:20643098


 [17] R. Sengupta, T.R. Billiar, V.E. Kagan, D.A. Stoyanovsky, Nitric oxide and thioredoxin type 1 modulate the activity of caspase 8 in HepG2 cells, Biochem Biophys Res Commun, 391 (2010) 1127-1130.PMC2812598


[18] Z. Huang, J. Jiang, N.A. Belikova, D.A. Stoyanovsky, V.E. Kagan, A.H. Mintz, Protection of normal brain cells from gamma-irradiation-induced apoptosis by a mitochondria-targeted triphenyl-phosphonium-nitroxide: a possible utility in glioblastoma therapy, J Neurooncol, 100 (2010) 1-8. PMID: 20835910


[19] R. Sengupta, T.R. Billiar, D.A. Stoyanovsky, Studies toward the analysis of S-nitrosoproteins, Org Biomol Chem, 7 (2009) 232-234. PMID:19109666


[20] R. Sengupta, T.R. Billiar, J.L. Atkins, V.E. Kagan, D.A. Stoyanovsky, Nitric oxide and dihydrolipoic acid modulate the activity of caspase 3 in HepG2 cells, FEBS Lett, 583 (2009) 3525-3530.PMC2789423


[21] M.R. Kapadia, J.W. Eng, Q. Jiang, D.A. Stoyanovsky, M.R. Kibbe, Nitric oxide regulates the 26S proteasome in vascular smooth muscle cells, Nitric Oxide, 20 (2009) 279-288. PMID:19233305


[22] V.E. Kagan, P. Wipf, D. Stoyanovsky, J.S. Greenberger, G. Borisenko, N.A. Belikova, N. Yanamala, A.K. Samhan Arias, M.A. Tungekar, J. Jiang, Y.Y. Tyurina, J. Ji, J. Klein-Seetharaman, B.R. Pitt, A.A. Shvedova, H. Bayir, Mitochondrial targeting of electron scavenging antioxidants: Regulation of selective oxidation vs random chain reactions, Adv Drug Deliv Rev, 61 (2009) 1375-1385.PMC2784017


[23] V.E. Kagan, H.A. Bayir, N.A. Belikova, O. Kapralov, Y.Y. Tyurina, V.A. Tyurin, J. Jiang, D.A. Stoyanovsky, P. Wipf, P.M. Kochanek, J.S. Greenberger, B. Pitt, A.A. Shvedova, G. Borisenko, Cytochrome c/cardiolipin relations in mitochondria: a kiss of death, Free Radic Biol Med, 46 (2009) 1439-1453.PMC2732771


[24] V.E. Kagan, A. Bayir, H. Bayir, D. Stoyanovsky, G.G. Borisenko, Y.Y. Tyurina, P. Wipf, J. Atkinson, J.S. Greenberger, R.S. Chapkin, N.A. Belikova, Mitochondria-targeted disruptors and inhibitors of cytochrome c/cardiolipin peroxidase complexes: a new strategy in anti-apoptotic drug discovery, Mol Nutr Food Res, 53 (2009) 104-114.PMC2659540


[25] J. Jiang, D.A. Stoyanovsky, N.A. Belikova, Y.Y. Tyurina, Q. Zhao, M.A. Tungekar, V. Kapralova, Z. Huang, A.H. Mintz, J.S. Greenberger, V.E. Kagan, A mitochondria-targeted triphenylphosphonium-conjugated nitroxide functions as a radioprotector/mitigator, Radiat Res, 172 (2009) 706-717.PMC2804962


[26] N.A. Belikova, J. Jiang, D.A. Stoyanovsky, A. Glumac, H. Bayir, J.S. Greenberger, V.E. Kagan, Mitochondria-targeted (2-hydroxyamino-vinyl)-triphenyl-phosphonium releases NO(.) and protects mouse embryonic cells against irradiation-induced apoptosis, FEBS Lett, 583 (2009) 1945-1950.PMC2696693

Detcho A. Stoyanovksy