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Andrew Amoscato, PhD

Res. Associate Professor, Environmental and Occupational Health

Contact

100 Techology Drive 15219
R-znvy: nnn0@cvgg.rqh
Primary Phone: 967-179-4430


Personal Statement



Dr. Amoscato has a wealth of experience in mass
spectrometry of lipids (lipidomics), peptides, proteins (proteomics), small
molecules (metabolomics) and imaging mass spectrometry. He has applied these areas
of mass spectrometric expertise to the study of lipid-protein/protein-protein
interactions, lipid alterations and phospholipid signaling during apoptosis and
autophagy. His main interests include mechanisms of apoptosis, autophagy and
ferroptosis, mitochondrial and redox biochemistry as well as the role of
oxidized lipids in these modes of cell death and. He is also interested in the role
of sphingolipids in radiation-induced apoptosis; the role of sphingolipids in
androgen ablation induced apoptosis of prostate cancer cells; the role of
lipids and lipid signaling in endothelial cell apoptosis and the role of lipid
DAMPS in inflammation and cancer.


Education

  • 1978, Long Island University, New York, NY, B.S. Biology and Chemistry
  • 1984, University of Texas Health Science Center at Houston, Graduate School of Biomedical Sciences, Houston, TX, Ph.D. Biochemistry

 


Selected Publications




Thomas RL, Matsko CM, Lotze MT, Amoscato AA. Mass spectrometric
identification of increased C16 ceramide levels during apoptosis. Jounral of
Biological Chemistry. 1999; 274:30580

Matsko CM, Hunter O, Rabinoqich H, Lotze MT, Amoscato AA. Alterations in mitochondrial
lipids during Fas- and radiation-induced apoptosis. Biochemical and Biophysical
Research Communications. 2001; 287:1112-20.

Ostrander DB, Sparagna GC, Amoscato AA, McMillin JB, Dowhan W. Decreased cardiolipin synthesis
corresponds with cytochrome c release in palmitate-induced cardiomyocyte
apoptosis. The Journal of biological chemistry. 2001 Oct 12; 276 (41):38061-7.
PMID: 11500520

Kanto, T. M., P. Kalinski, O. Hunter, M. T. Lotze and A. A. Amoscato. 2001. Ceramide mediates
tumor-induced dendritic cell apoptosis. 
J. Immunol. 167: 3773-84.

Eto M, Bennouna J, Hunter OC, Hershberger PA,
Kanto T, Johnson CS, Lotze MT, Amoscato
AA. C16 ceramide accumulates following androgen ablation in LNCaP prostate
cancer cells. The Prostate. 2003 Sep 15; 57 (1):66-79. PMID: 12886525.

Lee YJ, Amoscato
AA. TRAIL and ceramide. Vitamins and hormones. 2004; 67:229-55. PMID:
15110180.

  Kagan VE, Borisenko GG, Tyurina YY, Tyurin VA, Jiang J, Potapovich AI, Kini V, Amoscato AA, Fujii Y. Oxidative lipidomics of apoptosis: redox catalytic interactions of cytochrome c with
cardiolipin and phosphatidylserine. Free radical biology & medicine. 2004
Dec 15; 37 (12):1963-85. PMID: 15544916.
  
  Kagan VE, Tyurin VA, Jiang J, Tyurina YY, Ritov VB, Amoscato AA, Osipov AN, Belikova NA, Kapralov AA, Kini V, Vlasova II, Zhao Q, Zou M, Di P, Svistunenko DA, Kurnikov IV, Borisenko GG.
Cytochrome c acts as a cardiolipin oxygenase required for release of proapoptotic
factors. Nature chemical biology. 2005 Sep; 1 (4):223-32. PMID: 16408039.
  

Bayir H, Tyurin VA, Tyurina YY, Viner R, Ritov, V, Amoscato AA, Zhao Q, Zhang XJ,
Janesko-Feldman KL, Alexander H, Basova LV, Clark RS, Kochanek PM, Kagan VE. Selective
early cardiolipin peroxidation after traumatic brain injury: an oxidative
lipidomics analysis. Annals of neurology. 2007 Aug; 62 (2):154-69. PMID:
17685468.

Bayir H, Kapralov AA, Jiang J, Huang Z, Tyurina YY, Tyurin VA, Zhao Q, Belikova NA, Vlasova II, Maeda A, Zhu J, Na HM, Mastroberardino PG, Sparvero LJ, Amoscato
AA, Chu CT, Greenamyre JT, Kagan VE. Peroxidase mechanism of
lipid-dependent cross-linking of synuclein with cytochrome C: protection against
apoptosis versus delayed oxidative stress in Parkinson disease. The Journal of
biological chemistry. 2009 Jun 5; 284 (23):15951-69. PMCID: PMC2708890. PMID:
19351880.

Sparvero LJ, Amoscato AA, Kochanek PM, Pitt BR, Kagan VE, Bayir H.
Mass-spectrometry based oxidative lipidomics and lipid imaging: applications in
traumatic brain injury. Journal of neurochemistry. 2010 Dec; 115 (6):1322-36.
PMCID: PMC3285274. PMID: 20950335.

 

Atkinson J, Kapralov AA, Yanamala N, Tyurina YY, Amoscato AA, Pearce L, Peterson
J, Huang Z, Jiang J, Samhan-Arias AK, Maeda A, Feng W, Wasserloos K, Belikova
NA, Tyurin VA, Wang H, Fletcher J, Wang Y, Vlasova II, Klein-Seetharaman J,
Stoyanovsky DA, Bayîr H, Pitt BR, Epperly MW,7Greenberger JS, Kagan VE. A
mitochondria-targeted inhibitor of cytochrome c perxidase mitigates
radiation-induced death. Nature communications. 2011; 2:497. PMID: 21988913.

Samhan-Arias AK, Ji J, Demidova OM, Sparvero LJ, Feng W, Tyurin V, Tyurina YY, Epperly MW, Shvedova AA, Greenberger JS, Bayir H, Kagan VE, Amoscato AA.
Oxidized phospholipids as biomarkers of tissue and cell damage with a focus on
cardiolipin. Biochimica et biophysica acta. 2012 Mar 23. PMID: 22464971.


  Sparvero LJ, Amoscato AA, Dixon CE, Long
JB, Kochanek PM, Pitt BR, Bayir H, Kagan VE. Mapping of phospholipids by
MALDI imaging (MALDI-MSI): realities and expectations. Chemistry and physics
of lipids. 2012 Jul; 165 (5):545-62. PMCID: PMC3642772. PMID: 22692104.
  
  
 Ji J, Kline AE, Amoscato A, Samhan-Arias
AK, Sparvero LJ, Tyurin VA, Tyurina YY, Fink B, Manole MD, Puccio AM, Okonkwo
DO, Cheng JP, Alexander H, Clark RS, Kochanek PM, Wipf P, Kagan VE, Bayir H.
Lipidomics identifies cardiolipin oxidation as a mitochondrial target for
redox therapy of brain injury. Nature neuroscience. 2012 Oct; 15
(10):1407-13. PMID: 22922784.
  

Schlattner U, Tokarska-Schlattner M, Ramirez S, Tyurina YY, Amoscato AA,
Mohammadyani D, Huang Z, Jiang J, Yanamala N, Seffouh A, Boissan M, Epand RF,
Epand RM, Klein-Seetharaman J, Lacombe ML, Kagan VE. Dual function of
mitochondrial Nm23-H4 protein in phosphotransfer and intermembrane lipid
transfer: a cardiolipin-dependent switch. The Journal of biological chemistry.
2013 Jan 4; 288 (1):111-21. PMCID: PMC3537004. PMID: 23150663.

Chu, CT, Ji, J, Dagda, RK, Jiang, JF, Amoscato, AA, Kapralov, OK, Yanamala,
N, Klein-Seetharaman, J, Balasubramaniam, K, Borisenko, G, Huang, Z, Baty, C,
Watkins, S, Bayir, H and Kagan, VE Cardiolipin externalization as an
elimination signal for mammalian mitophagy. Nat. Cell Biol. 2013 15:1197-1205

Amoscato, AA, Sparvero, LJ, He, RR, Watkins, SW, Bayir, H and Kagan, VE. Imaging mass spectrometry of diversified cardiolipin molecular species in the brain. Analytical Chemistry 2014.
86:6587-95.


Stoyanovsky, DA, Sparvero, LJ, Amoscato, AA,
He, RR, Watkins, S, Pitt, BR, Bayir, H, and Kagan, VE Improved spatial
resolution of MALDI imaging of lipids in the brain by alkylated derivatives
of 2,5-dihydroxybenszoic acid. Rapid Comm. Mass Spectrom. 2014. 28:403-41


Cao, W, Ramakrishnan, R, Tuyrin, VA, Veglia, F, Condamine, T, Amoscato, A, Mohammadyani, D,
Johnson, JJ, Zhang, LM,  Judith Klein-Seetharaman, J, Celis, E,
Valerian E. Kagan, VE  and Gabrilovich, DI. Oxidized lipids block antigen cross-presentation by dendritic cells in cancer. J. Immunol. 2014
192:2920-31.


Jiang, J, Bakan , A, Kapralov, AA, Ishara, KI, Huang, Z, Amoscato, AA,  Peterson, J, Garapati, VK, Saxena, SK, Bayir, H, Atkinson,  J, Bahar, I,
Kagan, VE. Designing Inhibitors of Cytochrome c/Cardiolipin Peroxidase
Complexes:  Mitochondria-Targeted Imidazole-Substituted Fatty Acids.
Free Rad Biol Med. 2014. 71:221-230.


Tyurina, YY, Domingues, RM, Tyurin, VA, Maciel, E, Domingues, P, Amoscato, AA, Bayir, H, and Kagan, VE Characterization of cardiolipins and their oxidation products by LC-MS analysis. Chem. Phys. Lipids. 2014. 179:3-10.


Amoscato, AA,Sparvero, LJ, He, RR, Watkins, SW, Bayir, H and Kagan, VE. Imaging mass
spectrometry of diversified cardiolipin molecular species in the brain.
Analytical Chemistry 2014. 86:6587-95.


Mohammadyani, D., Tyurin, V. A., Amoscato, A. A.,
Gabrilovich, D. I., Klein-Seetharaman, J. and Kagan, V. E. Computational
Approaches to Understanding the Role of Oxidized Tri-Aclyglycerols in Suppression of Antigen Cross-Presentation in Cancer Biophysical
Journal 2015. 106:805a-806a.

Rolyan, H., Tyurina, Y., Hernandez, M., Amoscato,
A., Sparvero, L., Nmezi, B., Lu, Y., Estécio, M., Lin, K., Chen, J., He,
R., Pin Gong, Rigatti, L., Dupree J., Bayir, H., Kagan, V., Casaccia, P., and Padiath, Q., Defects of lipid synthesis underlie the age dependent
demyelination caused by lamin B1 Over expression. 2015. J. Neuroscience 35:12002-12017.

Mao,G., Qu, F., St. Croix, C., Tyurina, Y., Jiang, J., Huang, Z., Amoscato, A. A., Cheikhi, A., Bayir, H. and Kagan, V. Mitochondrial redox opto-lipidomics reveals selectively mono-oxygenated cardiolipins as pro-apoptotic death signals. ACS Chem. Biol. 2016. 11:530-40.



 




 

 

 




Andrew  Amoscato
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